ผลของวิธีการเก็บรักษาต่อปริมาณพฤกษเคมีและความสามารถในการต้านอนุมูลอิสระของน้ำฟักข้าว

บทคัดย่อ

Gac (Momordica cochinchinensis Spreng) has gained more interest due to its beneficial bioactive compounds and antioxidant capacity in the aril part of the fruit. Gac juice is therefore, manufactured as a functional drink to serve the consumer’s requirement of a natural supplement promoting good health condition. However, there is little information on the influence of light, temperature and storage duration on phytochemical content and antioxidant activity of gac juice. Hence, the objective of this study was to investigate the effect of light, temperature and time on the content of phytochemical including total phenolic compound, flavonoid, lycopene and ?-carotene as well as the antioxidant activity of gac juice. The total phenolic content was determined using FolinCiocalteu method. The total flavonoid content was accessed by colorimetric method. The quantification of lycopene and ?-carotene were carried out using spectrophotometer at 470 and 450 nm, respectively. For antioxidant capacity determination, DPPH and FRAP assays were used. All analyses were carried out for fresh juices and after storage at 4, 25 and 37?C for 2, 4, 6, 8, 10 and 12 weeks in amber and clear glass bottles. The result revealed that phytochemicals were affected by light, time and temperature of storage. The decrease in the amount of phytochemicals upon the storage corresponded to the decrease in the antioxidant activity of gac juice. In addition, the quantity of bioactive compounds and antioxidant activity were obviously reduced upon the storage duration. The optimal condition to retain the highest phytochemical and antioxidant activity was to keep the juice in amber bottle at 4?C for 2 weeks.

คำสำคัญ