ศึกษาโปรตีนและเปปไทด์ของสารหลั่งและเซลล์จากรังไข่สุกรเพื่อความเป็นไปได้ในการใช้เป็นส่วนผสมในผลิตภัณฑ์เสริมความงาม

บทคัดย่อ

Our project studied morphology of oocytes, follicle cells and follicular fluid proteins of Large White pig. Porcineovaries collected from local slaughter house in NakhonPathom Province. The porcine oocytes and follicular fluid of healthy small follicles (1-2 and 3-4 mm), medium follicles (5-6 mm in diameters) and large follicles (7-8 mm and 10 mm in diameter) were aspirated and collected from the ovaries by sterile technique. Then, the oocytes andthe follicle cellswere separated from the fluid. The oocytes were round shape and surrounded by zonapellucida with numerous layers of cumulus cells. Based on the number of cumulus celllayers surrounding oocytes, the oocyteswere classified into 5 types, which were intact-, multi-, partial- cumulus layer oocyte, completely denuded oocyte and degenerative oocyte.The collected oocytes showed high percentages of intact- and multi- cumulus cell layers in the small follicles (54.05%) medium follicles (56.94%) and large follicles (56.52%) which have high potential to develop into mature oocytes in vitro. The proteins from follicular fluid of 3 size follicles were separated by SDS-PAGE and LC/MS/MS. The molecular weight of follicular fluid proteins from the small follicles were ranging from 27, 62, 70, 80, 115, 140, >220 kDa. Interestingly, protein bands of 70 and 80 kDa played an important role in decreasing metaphase I of oocyte maturation. The follicular fluid protein from medium and large follicle contained 50, 65, 75, 90, 95, 110, 120, 160, 180, 190 and >220 kDa. All of these proteins played important roles in promoting and regulatinggrowth and development of oocytes and ovulation. This finding was an initial tool for in vitro testing and applied biotechnology research and may be developed as part of ingredients for cosmetic products.

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