การยับยั้งฤทธิ์ของสารอนุมูลอิสระด้วยสารสกัดจากใบมะรุม

บทคัดย่อ

Chronic inflammatory conditions lead to the generation of excessive reactive oxygen species which can give rise to T cell hyporesponsiveness. Thus there has been an interest to target these mediators with nutrients which have anti-oxidant properties. Accordingly Moringa oleifera extract has been reported to have anti-inflammatory and anti-oxidant properties. We have now examined the effects of Moringa oleifera Lam. on the depressed human T-cell responses, (IL-2 and IL-2 mRNA production) and DNA damage (quantifying the oxidized derivative of deoxyguanosine, 8-OHdG) induced oxidative stress. T-cells treated with oxidative substances, uric acid, aluminum chloride, hydrogen peroxide, and ultraviolet irradiation showed marked reduction in IL-2 production, which was prevented by pre-treating the cells with Moringa extract. These effects correlated with its prevention of the down-regulation of IL2 gene expression and a reduction in the DNA damage caused by oxidative stress. Thus the ability of Moringa extract to reduce inflammation is likely to relate to its ability to prevent T-cell hyporesponsiveness experienced in inflammatory diseases, through an action at the pre-transcriptional level.

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