การผลิตพอลีไฮดรอกซีอัลคาโนเอทโดยการหมักกากปาล์มน้ำมันด้วยจุลินทรีย์ที่คัดเลือกได้จากดินบริเวณบ่อบำบัดน้ำเสียโรงงานสกัดน้ำมันปาล์ม

บทคัดย่อ

Palm is the important industrial drop for production of an edible oil. The vegetable oil producing process can release much amount of waste and waste water that have effected on environment problem, that cause by organic compound as starch and lipid, which is the carbon source of bacteria for produce “PHAs”. PHAs is the raw material for producing of Bioplastic. Thus, the objective of this research was isolation and screening for bacteria that can produce PHAs from waste of palm oil. The waste water and soil were collected from 6 palm oil industries in Krabi, Surat Thani and Chumphon provinces. Seven isolates of lipid granule producing bacteria were discovered. The cultivated bacteria were estimated, lipid granules in the cell of bacteria that may be produced PHAs were stained with Sudan Black B dyeing and detected with microscope. Seven isolates were found that they showed the lipid granules in cells and cultivates these isolates in the “Nitrogen limiting medium” for 48 hours. Then, confirmation for PHAs synthesis in their cells was approved by Nile Blue A dye staining to inspect the fluorescence of the lipid granules with the Fluorescence Microscope. This was compared to the positive control bacteria. There were found 3 isolates, NKBT02, NKBT07 and NKBT10. However, the results showed that NBKT10 produced 22- 37% (w/w) of PHAs when the steamed oil palm (mesocarp) mill added 1% (w/w) of glucose was used as carbon and nitrogen sources with the aeration.

คำสำคัญ