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Dry eye disease (DED) is one of the most common eye diseases globally and affects mainly the elderly. In severe DED, damage of functional LG tissue occurs, resulting in a deficit of aqueous tears, corneal inflammation and ulcers, which can lead to a loss of vision in the long-term. Yet, no curative treatments exist for DED and investigations to regenerate the LG are currently underway. One promising methodology to induce LG regeneration uses mesenchymal stem cell (MSC) populations and/or their secretome. These MSC populations can be found in multiple tissues such as bone marrow, umbilical cord, adipose tissue, as well as in the dental pulp as dental pulp stem cells (DPSC). Recently, our research group has successfully bio-engineered “mini” salivary glands - exocrine glands similar to the LG - from the bioassembly of human DPSC via magnetic organoid bioprinting. These bioprinting strategies are on the rise due to their effectiveness on the prompt and easy assembly of cells into three-dimensional viable tissues and organoids (“mini-organs”). Hence, the main goal of this research project is to develop a biotechnology platform for the biofabrication of LG organoids through magnetic bioprinting of hDPSC-derived cells. Once biofabrication is completed, the LG organoid can target many research and biomedical applications from in vitro drug testing and disease modeling to cell-based and secretome-based therapies for LG regeneration purposes in DED patients.